MersV1, France, Analysis, bibRecord, 000237

Structural organisation of the head-to-tail interface of a bacterial virus

Identifieur interne : 000237 ( France/Analysis ); précédent : 000236; suivant : 000238

Structural organisation of the head-to-tail interface of a bacterial virus

Auteurs : Rudi Lurz [Allemagne] ; Elena V. Orlova [Allemagne] ; Dirk Günther [Allemagne] ; Prakash Dube [Royaume-Uni] ; Anja Dröge [Allemagne] ; Frank Weise [Allemagne] ; Marin Van Heel [Royaume-Uni] ; Paulo Tavares [France, Allemagne]

Source :

Journal of Molecular Biology [ 0022-2836 ] ; 2001.

RBID : ISTEX:E2D7D1C0E2933500C7ACFF805BE2CD874E8A8F09

English descriptors

Abstract

Abstract: In tailed icosahedral bacteriophages the connection between the 5-fold symmetric environment of the portal vertex in the capsid and the 6-fold symmetric phage tail is formed by a complex interface structure. The current study provides the detailed analysis of the assembly and structural organisation of such an interface within a phage having a long tail. The region of the interface assembled as part of the viral capsid (connector) was purified from DNA-filled capsids of the Bacillus subtilis bacteriophage SPP1. It is composed of oligomers of gp6, the SPP1 portal protein, of gp15, and of gp16. The SPP1 connector structure is formed by a mushroom-like portal protein whose cap faces the interior of the viral capsid in intact virions, an annular structure below the stem of the mushroom, and a second narrower annulus that is in direct contact with the helical tail extremity. The layered arrangement correlates to the stacking of gp6, gp15, and gp16 on top of the tail. The gp16 ring is exposed to the virion outside. During SPP1 morphogenesis, gp6 participates in the procapsid assembly reaction, an early step in the assembly pathway, while gp15 and gp16 bind to the capsid portal vertex after viral chromosome encapsidation. gp16 is processed during or after tail attachment to the connector region. The portal protein gp6 has 12-fold cyclical symmetry in the connector structure, whereas assembly-naı̈ve gp6 exhibits 13-fold symmetry. We propose that it is the interaction of gp6 with other viral morphogenetic proteins that drives its assembly into the 12-mer state.

Url:

https://api.istex.fr/ark:/67375/6H6-137S7R5X-P/fulltext.pdf

DOI: 10.1006/jmbi.2001.4800

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000535
to stream Istex, to step Curation: 000535
to stream Istex, to step Checkpoint: 000E32
to stream Main, to step Merge: 003548
to stream Main, to step Curation: 003510
to stream Main, to step Exploration: 003510
to stream France, to step Extraction: 000237

Links to Exploration step

ISTEX:E2D7D1C0E2933500C7ACFF805BE2CD874E8A8F09

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structural organisation of the head-to-tail interface of a bacterial virus</title>
<author><name sortKey="Lurz, Rudi" sort="Lurz, Rudi" uniqKey="Lurz R" first="Rudi" last="Lurz">Rudi Lurz</name>
</author>
<author><name sortKey="Orlova, Elena V" sort="Orlova, Elena V" uniqKey="Orlova E" first="Elena V." last="Orlova">Elena V. Orlova</name>
</author>
<author><name sortKey="Gunther, Dirk" sort="Gunther, Dirk" uniqKey="Gunther D" first="Dirk" last="Günther">Dirk Günther</name>
</author>
<author><name sortKey="Dube, Prakash" sort="Dube, Prakash" uniqKey="Dube P" first="Prakash" last="Dube">Prakash Dube</name>
</author>
<author><name sortKey="Droge, Anja" sort="Droge, Anja" uniqKey="Droge A" first="Anja" last="Dröge">Anja Dröge</name>
</author>
<author><name sortKey="Weise, Frank" sort="Weise, Frank" uniqKey="Weise F" first="Frank" last="Weise">Frank Weise</name>
</author>
<author><name sortKey="Van Heel, Marin" sort="Van Heel, Marin" uniqKey="Van Heel M" first="Marin" last="Van Heel">Marin Van Heel</name>
</author>
<author><name sortKey="Tavares, Paulo" sort="Tavares, Paulo" uniqKey="Tavares P" first="Paulo" last="Tavares">Paulo Tavares</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:E2D7D1C0E2933500C7ACFF805BE2CD874E8A8F09</idno>
<date when="2001" year="2001">2001</date>
<idno type="doi">10.1006/jmbi.2001.4800</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-137S7R5X-P/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000535</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000535</idno>
<idno type="wicri:Area/Istex/Curation">000535</idno>
<idno type="wicri:Area/Istex/Checkpoint">000E32</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000E32</idno>
<idno type="wicri:doubleKey">0022-2836:2001:Lurz R:structural:organisation:of</idno>
<idno type="wicri:Area/Main/Merge">003548</idno>
<idno type="wicri:Area/Main/Curation">003510</idno>
<idno type="wicri:Area/Main/Exploration">003510</idno>
<idno type="wicri:Area/France/Extraction">000237</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Structural organisation of the head-to-tail interface of a bacterial virus</title>
<author><name sortKey="Lurz, Rudi" sort="Lurz, Rudi" uniqKey="Lurz R" first="Rudi" last="Lurz">Rudi Lurz</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Max-Planck-Institut für Molekulare Genetik, Ihnestraße 73, 14195 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Orlova, Elena V" sort="Orlova, Elena V" uniqKey="Orlova E" first="Elena V." last="Orlova">Elena V. Orlova</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Max-Planck-Institut für Molekulare Genetik, Ihnestraße 73, 14195 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gunther, Dirk" sort="Gunther, Dirk" uniqKey="Gunther D" first="Dirk" last="Günther">Dirk Günther</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Max-Planck-Institut für Molekulare Genetik, Ihnestraße 73, 14195 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dube, Prakash" sort="Dube, Prakash" uniqKey="Dube P" first="Prakash" last="Dube">Prakash Dube</name>
<affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Imperial College of Science Technology and Medicine Wolfson Laboratories Department of Biochemistry, London SW7 2AY</wicri:regionArea>
<wicri:noRegion>London SW7 2AY</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Droge, Anja" sort="Droge, Anja" uniqKey="Droge A" first="Anja" last="Dröge">Anja Dröge</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Max-Planck-Institut für Molekulare Genetik, Ihnestraße 73, 14195 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Weise, Frank" sort="Weise, Frank" uniqKey="Weise F" first="Frank" last="Weise">Frank Weise</name>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Max-Planck-Institut für Molekulare Genetik, Ihnestraße 73, 14195 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Van Heel, Marin" sort="Van Heel, Marin" uniqKey="Van Heel M" first="Marin" last="Van Heel">Marin Van Heel</name>
<affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Imperial College of Science Technology and Medicine Wolfson Laboratories Department of Biochemistry, London SW7 2AY</wicri:regionArea>
<wicri:noRegion>London SW7 2AY</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Tavares, Paulo" sort="Tavares, Paulo" uniqKey="Tavares P" first="Paulo" last="Tavares">Paulo Tavares</name>
<affiliation wicri:level="1"><country wicri:rule="url">France</country>
</affiliation>
<affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Max-Planck-Institut für Molekulare Genetik, Ihnestraße 73, 14195 Berlin</wicri:regionArea>
<placeName><region type="land" nuts="3">Berlin</region>
<settlement type="city">Berlin</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Journal of Molecular Biology</title>
<title level="j" type="abbrev">YJMBI</title>
<idno type="ISSN">0022-2836</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="2001">2001</date>
<biblScope unit="volume">310</biblScope>
<biblScope unit="issue">5</biblScope>
<biblScope unit="page" from="1027">1027</biblScope>
<biblScope unit="page" to="1037">1037</biblScope>
</imprint>
<idno type="ISSN">0022-2836</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0022-2836</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>EM</term>
<term>Hisgp15</term>
<term>Ig</term>
<term>bacteriophage SPP1</term>
<term>connector</term>
<term>cyclical oligomers</term>
<term>gp</term>
<term>portal protein</term>
<term>symmetry</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Bacillus subtilis bacteriophage spp1</term>
<term>Bacteriophage</term>
<term>Bacteriophage lambda</term>
<term>Bacteriophage spp1</term>
<term>Bacteriophage spp1 connector</term>
<term>Bacteriophage spp1 connector figure</term>
<term>Bacteriophage spp1 portal protein</term>
<term>Biol</term>
<term>Capsid</term>
<term>Carrascosa</term>
<term>Characteristic views</term>
<term>Class averages</term>
<term>Connector</term>
<term>Connector particles</term>
<term>Connector preparation</term>
<term>Connector proteins</term>
<term>Connector region</term>
<term>Connector structure</term>
<term>Cyclical</term>
<term>Dube</term>
<term>Edta</term>
<term>Eigenimage number</term>
<term>Electron microscope</term>
<term>Electron microscopy</term>
<term>Head completion proteins</term>
<term>Helical</term>
<term>Helical tail</term>
<term>Icosahedral capsid</term>
<term>Image processing</term>
<term>Individual particles</term>
<term>Interface</term>
<term>Lurz</term>
<term>Lysis buffer</term>
<term>Mature virions</term>
<term>Morphogenesis</term>
<term>Organisation</term>
<term>Packaging machinery</term>
<term>Phage</term>
<term>Phage particle</term>
<term>Portal</term>
<term>Portal protein</term>
<term>Portal vertex</term>
<term>Procapsid</term>
<term>Procapsid assembly</term>
<term>Protein</term>
<term>Spp1</term>
<term>Spp1 connector</term>
<term>Spp1 interface</term>
<term>Spp1 particles</term>
<term>Spp1 portal protein</term>
<term>Spp1 virions</term>
<term>Structural organisation</term>
<term>Subunit</term>
<term>Tail structures</term>
<term>Tavares</term>
<term>Unpublished results</term>
<term>Viral</term>
<term>Viral capsid</term>
<term>Virion</term>
<term>Western blot</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: In tailed icosahedral bacteriophages the connection between the 5-fold symmetric environment of the portal vertex in the capsid and the 6-fold symmetric phage tail is formed by a complex interface structure. The current study provides the detailed analysis of the assembly and structural organisation of such an interface within a phage having a long tail. The region of the interface assembled as part of the viral capsid (connector) was purified from DNA-filled capsids of the Bacillus subtilis bacteriophage SPP1. It is composed of oligomers of gp6, the SPP1 portal protein, of gp15, and of gp16. The SPP1 connector structure is formed by a mushroom-like portal protein whose cap faces the interior of the viral capsid in intact virions, an annular structure below the stem of the mushroom, and a second narrower annulus that is in direct contact with the helical tail extremity. The layered arrangement correlates to the stacking of gp6, gp15, and gp16 on top of the tail. The gp16 ring is exposed to the virion outside. During SPP1 morphogenesis, gp6 participates in the procapsid assembly reaction, an early step in the assembly pathway, while gp15 and gp16 bind to the capsid portal vertex after viral chromosome encapsidation. gp16 is processed during or after tail attachment to the connector region. The portal protein gp6 has 12-fold cyclical symmetry in the connector structure, whereas assembly-naı̈ve gp6 exhibits 13-fold symmetry. We propose that it is the interaction of gp6 with other viral morphogenetic proteins that drives its assembly into the 12-mer state.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
<li>France</li>
<li>Royaume-Uni</li>
</country>
<region><li>Berlin</li>
</region>
<settlement><li>Berlin</li>
</settlement>
</list>
<tree><country name="Allemagne"><region name="Berlin"><name sortKey="Lurz, Rudi" sort="Lurz, Rudi" uniqKey="Lurz R" first="Rudi" last="Lurz">Rudi Lurz</name>
</region>
<name sortKey="Droge, Anja" sort="Droge, Anja" uniqKey="Droge A" first="Anja" last="Dröge">Anja Dröge</name>
<name sortKey="Gunther, Dirk" sort="Gunther, Dirk" uniqKey="Gunther D" first="Dirk" last="Günther">Dirk Günther</name>
<name sortKey="Orlova, Elena V" sort="Orlova, Elena V" uniqKey="Orlova E" first="Elena V." last="Orlova">Elena V. Orlova</name>
<name sortKey="Tavares, Paulo" sort="Tavares, Paulo" uniqKey="Tavares P" first="Paulo" last="Tavares">Paulo Tavares</name>
<name sortKey="Weise, Frank" sort="Weise, Frank" uniqKey="Weise F" first="Frank" last="Weise">Frank Weise</name>
</country>
<country name="Royaume-Uni"><noRegion><name sortKey="Dube, Prakash" sort="Dube, Prakash" uniqKey="Dube P" first="Prakash" last="Dube">Prakash Dube</name>
</noRegion>
<name sortKey="Van Heel, Marin" sort="Van Heel, Marin" uniqKey="Van Heel M" first="Marin" last="Van Heel">Marin Van Heel</name>
</country>
<country name="France"><noRegion><name sortKey="Tavares, Paulo" sort="Tavares, Paulo" uniqKey="Tavares P" first="Paulo" last="Tavares">Paulo Tavares</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/France/Analysis

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000237 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000237 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    France
   |étape=   Analysis
   |type=    RBID
   |clé=     ISTEX:E2D7D1C0E2933500C7ACFF805BE2CD874E8A8F09
   |texte=   Structural organisation of the head-to-tail interface of a bacterial virus
}}

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

Structural organisation of the head-to-tail interface of a bacterial virus

Structural organisation of the head-to-tail interface of a bacterial virus

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri